Public Dissemination Event
El Puerto de Santa María, 3rd October 2019
Characterization of a new microalgae extract with anti-inflammatory activity in human fibroblasts
Israel Guerrero-Cózar1; Sophia Letsiou2; Lalia Mantecón3; Konstantinos Gardikis2; Manuel Manchado1,2
1IFAPA Centro El Toruño, Junta de Andalucía, Camino Tiro Pichón s/n, 11500 El Puerto de Santa María, Spain
2Research and Development Department, APIVITA S.A., Athens, Greece
3Fitoplanton Marino S.L., 11500, El Puerto de Santa María, Spain
4“Crecimiento Azul", Centro IFAPA El Toruño, Unidad Asociada al CSIC”
Microalgal extracts are an important source of bioactive compounds with antioxidant and anti-inflammatory functions that can be used as supplements in aquaculture and cosmetics. In this study, an aqueous extract (MA) from the diatom Phaeodactylum tricornutum was prepared and they were microencapsulated using hydroxypropyl cyclodextrin (CD) and hydrolysed rice flour (HRF) as carriers to enhance the bioavailability and bioactivity. As a control for bioactivity, yeast beta-glucans (Y) were used. All the molecules were UV-irradiated to reduce microbiological contamination. To assess the effects on cell viability, the crude extracts and their encapsulates were assayed in normal fibroblast human cells for 24h. While HRF encapsulates and crude extracts did not clearly improve cell viability, the CD encapsulates, mainly CD-Y, increased it. The PCA analysis using the expression profiles of 11 genes indicated a clear separation of CD-MA samples from the CD carrier. Genes related to cell proliferation (HGF) and antioxidant defences (NRF2, GPX1, SOD1) were heightened and pro-inflammatory cytokines (IL1B) were reduced in CD-MA samples. An effect of the CD carrier was observed in some genes. To test the potential anti-inflammatory capacity of this extract, cells were pre-treated with crude extracts and encapsulates at the same doses as indicated above for 24h and then exposed to bacterial lipopolysaccharide (LPS) The expression data indicated that untreated controls treated with LPS highly activated the expression of proinflammatory cytokines and antioxidant defences. When the protective effects of extracts were evaluated using MTT, the HRF encapsulates, mainly HRF-MA, and crude extracts (MA and Y) increased cell viability. The expression profiles indicated that HRF-carrier itself mitigated inflammatory response induced by LPS and promoted cell proliferation but these effects were highly heightened by HRF-MA. The crude extracts MA also promoted an anti-inflammatory and antioxidant state and activated cell proliferation indicating the bioactivity of this extract. All these data indicate the potential of this new extract to be used in cosmetics to reduce skin inflammation and the use of encapsulates.
This research has been funded by the project Algae4A&B from the UE H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 691102 The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the abstract.