Public Dissemination Event

El Puerto de Santa María, 3rd October 2019

Comparative in vitro models for cosmeceutical testing: the utility of fish cell lines



João CR Cardoso1; Rute C Félix1; Sophia Letsiou2; Deborah Power1

1Centre of Marine Sciences (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal;

2Research and Development Department, APIVITA S.A., Athens, Greece



The maintenance of skin integrity and retardation of skin aging are targets of the cosmetics industry. Considerable attention has been dedicated to the development of novel cosmeceutical products to slow down skin aging and to improve skin heath and structure. Animal testing for cosmeceuticals is banned in the European Union and to screen for novel active compounds and assess their potential beneficial effects on human skin health, mammalian in vitro non-animal testing models using primary dermal cell lines (eg. fibroblasts and keratinocytes) and skin 3D models have been developed. Recently studies on fish skin have highlighted the need for relevant model for dermatology due to the skin unique characteristics. In contrast to mammalian skin, the outermost surface of fish skin is composed of live epidermal cells and a layer of dead cornified cells is not found. The fish skin model is very interesting as it has a high regenerative capacity, does not wrinkle or scar and the physiological systems that control skin physiology (endocrine and immune systems) are less complex than in human. The aim of this study was to develop primary dermal cell cultures of fish skin fibroblasts and keratinocytes from marine aquaculture fish to assess their utility for human cosmeceutical screening.



This research has been funded by MSCA-RISE project 691102 (Algae4A&B) and the institutional project CCMAR/Multi/04326/2013 from the Portuguese Foundation for Science and Technology (FCT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the abstract.